---
title: High-Content Screening and High-Throughput RNA Sequencing using hiPSC-CMs for the Assessment of Functional and Structural Cardiotoxicity
description: hide gateway
image: https://news.cyprotex.com/hubfs/Sciencepool/Sciencepool%20images/Shutterstock%20images/Heart_shutterstock_1481125922.png
---

[![Cyprotex | The Leading ADME-Tox Specialist](https://news.cyprotex.com/hs-fs/hubfs/cyprotex%20logo.png?width=224&height=62&name=cyprotex%20logo.png "Cyprotex | The Leading ADME-Tox Specialist")](https://www.cyprotex.com/)

# Science Pool

# High-Content Screening and High-Throughput RNA Sequencing using hiPSC-CMs for the Assessment of Functional and Structural Cardiotoxicity

Posted by [Evotec](https://news.cyprotex.com/sciencepool/author/evotec) on Jul 20, 2023 3:56:14 PM

Drug-induced cardiotoxicity may result from a functional change in cardiac electrophysiology (acute alteration of the mechanical function of the myocardium) and/or from a structural change, resulting in damage to the cardiac tissue. In our research, the effects of 42 reference compounds in human-induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) were investigated in a combined risk assessment strategy. Functional cardiotoxicity was evaluated through kinetic monitoring of calcium transients (CaT), while structural morphology changes and gross cytotoxicity were assessed using high-content imaging (HCI) and cellular ATP measurements. In addition, whole genome high-throughput RNA-sequencing (ScreenSeq) was performed in matched-sister plates. Data were analysed to determine differentially expressed genes (DEGs) and any associated perturbed pathways.

 

[Download](https://news.cyprotex.com/hubfs/Sciencepool/Posters/Cyprotex%20posters/HC-screening-and-HT-RNA-sequencing-using-hiPSC-CMs-for-cardiotoxicity-SOT-2023-poster.pdf)

 

 

 

 

 Tags: [Posters](https://news.cyprotex.com/sciencepool/tag/posters), [Toxicology & Safety](https://news.cyprotex.com/sciencepool/tag/toxicology-safety)