Mass spectrometry (MS)-based ubiquitinomics allows a system-level understanding of ubiquitin signalling.
In this publication, we focus on:
- a background to ubiquitinome profiling
- presentation of a scalable and robust workflow for deep and precise in vivo ubiquitinome profiling using DIA-MS (data-independent acquisition mass spectrometry) and neural network based data processing
- comprehensive mapping of substrates of deubiquitinase USP7, an anticancer drug target known to regulate tumour suppressor p53
- application of the method including rapid mode of action profiling of candidate drugs targeting deubiquitinases or ubiquitin ligases