Reactive metabolites play a role in drug-induced toxicity. Early stage in vitro screening for electrophilic reactive metabolite formation involves the use of trapping agents such as glutathione (GSH) in the presence of a drug metabolising system.
In this poster, we focus on:
- the use of an alternative GSH trapping method using combined stable labelled GSH (GSH-13C2,15N) with unlabelled GSH
- the advantages of the stable labelled GSH method over common approaches for the detection of GSH conjugation such as GSH neutral loss post acquisition
- analytical conditions including the HPLC and mass spectrometric methods
- validation data for a set of literature compounds
Read our poster to learn more about our research!