Science Pool

Enhancing throughput while reducing footprint is a recurring requirement in sample management. With the new Smartcel-2 from Flexible Lab Solutions, we have significantly boosted our capability to prepare assay-ready plates in a serial dilution format. This double-deck automation system features a Bravo from Agilent for high-volume serial dilution plate preparation and a mosquito LV from SPT Labtech for nanoliter dispensing steps. Several peripheral devices, including a peeler, sealer, centrifuge, and plate storage, enable the automation cell to operate around the clock. 

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With the growing interest in Biologics within the drug discovery field, the Evotec Sample Management teams have been asked to offer their platforms and expertise to enhance throughput and timelines by establishing robust, high-quality processes in aqueous solvents. While extensive experience with DMSO-based solvents was leveraged, the significantly different properties of the water-based solvents raised new questions regarding the handling of source and destination plates at Sample Management.
 

To address these challenges, comprehensive tests were conducted, focusing particularly on evaporation and the establishment of a device verification process. This poster presents these processes and provides an overview of the results.

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To establish a seamless connection between the CyBio Well vario liquid handling system and the Mosaic interface using the new JFM integration. This integration aims to enhance the flexibility, reliability, and user-friendliness of liquid handling operations.

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This poster relates to enhancing dose selection in phase I cancer trials.

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This poster describes the rapid conversion of an intensified fed-batch antibody manufacturing process to an integrated continuous biomanufacturing process using the Just-Evotec Biologics platform, resulting in several key project accomplishments: 

• Mitigation of upstream IFB challenges
• Significant productivity increase
• Short development time
• Minimal risk from changes in product quality

These results demonstrate that the rapid conversion of fed-batch processes for monoclonal antibodies to an integrated continuous biomanufacturing process can be achieved with a robust ICB platform. This supports the biotherapeutics industry’s need to quickly adapt to changing clinical and business circumstances.

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Biologics manufacturing typically uses engineered Chinese Hamster Ovary (CHO) cells to produce folded and glycosylated antibodies. Determining the optimum conditions to grow and maintain cell culture often requires considerable time and effort.

A quantitative understanding of cell metabolism through an analysis of cell culture metabolites can enable optimization growth conditions for improved titles or increased perfusion duration. Mass spectrometry is the optimum tool for metabolite measurement, however, transforming raw data into accurate quantitative measurement requires both expertise and extensive sample preparation.

In this poster we demonstrate the ability of simple sample preparation using universal calibrators and a novel machine learning algorithm to rapidly provide biological insight into bioprocessing samples taken from perfusion cell cultures.

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High-throughput screening methodologies have accelerated downstream development for monoclonal antibodies by enabling parallelized evaluation of chromatographic resins across a range of conditions. However, scientists must now interpret results in a meaningful and consistent way.

Learn how Just - Evotec Biologics' Downstream Data Browser automates visualization of high-throughput datasets, fits response surface statistical models, standardizes report results from a high-throughput screening method and facilitates comparison across molecules allowing the accelerated development of continuous biomanufacturing processes.

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Endocrine disruptors, which can be either natural or man-made, interfere with the normal actions of hormones in the body. This can result in serious health issues such as cancer, birth defects and developmental disorders. Low cost screening models are needed to flag chemicals as potential endocrine disruptors at an early stage.

In this poster, we focus on:

• the development of a new high throughput screening panel for chemicals to detect potential endocrine disruptors
• the comparison between endocrine receptor activation assays expressing the human androgen receptor, the human estrogen receptor or the human thyroid receptor, and hormone receptor competitor binding assays using fluorescence polarisation.
• the relationship between the literature and the data generated in the assays.

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Enhancing Single-Pass TFF for Antibody Therapeutics Manufacturing

Single-Pass Tangential Flow Filtration (TFF) plays a pivotal role in enabling fully end-to-end continuous manufacturing of antibody therapeutics. In this poster, we delve into the study of two distinct SP-TFF membrane configurations to determine which one is most effective for clinical and commercial production.

Key Findings:

1.    Concentration Factor Achievement: Both tested configurations successfully achieved the required concentration factor without encountering fouling issues.
2.    Shear Forces Mitigation: Neither setup generated significant shear forces that could harm the antibody product.
3.    Operational Success at Low Feed Flux: Both configurations demonstrated successful operations even under low feed flux conditions, reinforcing their suitability for large-scale manufacturing.

Future Directions: Our team of scientists and engineers will continue their investigation, exploring various load challenges, system perturbations, and methods for in-line concentration measurements. These efforts aim to enhance the robustness and efficiency of our manufacturing processes.

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Food additive testing is essential to ensure human safety during consumption. Early stage genotoxicity screening is an efficient way of triaging additives to ensure only the safest additives are taken forward for more rigorous testing.

In this poster, we focus on:

• the development and validation of a new early stage genotoxicity approach for food additives
• the use of a panel of in vitro assays for detecting clastogens (pH2AX) and aneugens (pH3) as well as micronuclei formation (MNT)

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